Respuesta :
4. sgRNA binds Cas9
3. Cas9/sgRNA complex binds PAM
5. Cas9 unzips DNA
1. small guide RNA (sgRNA) hybridizes with the target DNA
2. Cas9 cuts DNA
Every live cell has a genome (DNA). DNA is a double helix structure kept together by the complementary base pairing rule, which states that adenine always pairs with thymine and guanine always pairs with cytosine.
Researchers have discovered thousands of genes that might cause sickness in our bodies. Changing these genes in our bodies is difficult, but it is now achievable thanks to the CRISPR method.
CRISPR:
This method is based on natural system used by bacteria against the DNA of bacteriophages by activating CAS9 protein which due to guide RNA recognizes the target DNA and cut it into pieces.
Mechanism of CRISPR:
Initially, tiny guide RNA forms a complex with the CAS9 endonuclease protein.
This results in the formation of a Cas9/sgRNA complex, which subsequently binds the PAM sequence found inside the target DNA.
Cas9 unzips DNA when it binds to the PAM sequence.
The CAS9 complex contains small guide RNA (sgRNA), which hybridizes with the target DNA.
Cas9, which chops the target DNA, detects this hybridization.
Learn more about to CRISPR visit here;
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