The substrate concentration at which a reaction progresses at half its maximum velocity is known as the Michaelis constant, or Km. The affinities are most precisely represented by this constant.
A high Michaelis constant: what does that mean?
The Michaelis constant is a measure of the substrate's affinity for the enzyme; a small value implies high affinity, suggesting that the rate will approach with lower than those processes with a higher value. Its value is numerically equivalent to the at which the reaction rate is at half-maximum.
The significance of a low Michaelis-Menten constant
The enzyme's affinity for the molecules of the substrate is represented by the Michaelis-Menten constant. Less substrate concentration will be required if the enzyme has a high affinity for the substrate.
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