The Sanger approach makes use of dideoxynucleotides or ddNTPs similarly to ordinary deoxynucleotides (dNTPs) within side the response. ddNTPs bring about termination of the DNA strand due to the fact ddNTPs lack the 3'-OH institution required for phosphodiester bond formation among nucleotides.
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Dideoxynucleotides are chain-elongating inhibitors of DNA polymerase, used within side the Sanger technique for DNA sequencing. They are also referred to as 2',3' because of the truth every the 2' and 3' positions on the ribose lack hydroxyl groups, and are abbreviated as ddNTPs (ddGTP, ddATP, ddTTP and ddCTP). The Sanger technique is used to increase a goal section of DNA, in order that the DNA series may be decided precisely. The incorporation of ddNTPs within side the response valves are in reality used to terminate the synthesis of a developing DNA strand, ensuing in partly replicated DNA fragments.
This is due to the fact DNA polymerase calls for the 3' OH institution of the developing chain and the 5' phosphate institution of the incoming dNTP to create a phosphodiester bond.
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