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Each sister chromatid uses a protein complex termed the kinetochore to connect to spindle microtubules at the centromere.

Sister chromatids are bound to one another during mitotic prometaphase, but why?

Cohesion is necessary for sister chromatids to stay together during mitosis and meiosis until separation is about to occur at anaphase. Cohesins are multiprotein component complexes that help create cohesion during DNA replication prior to mitosis and meiosis.

During prometaphase, where do microtubules bind to sister chromatids?

kinetochores

The production of a protein structure known as a kinetochore around the centromere, the central region connecting the sister chromatids, is another significant process that occurs during prometaphase. Kinetochore microtubules are lengthy protein filaments that extend from the cell's poles at each end and connect to the kinetochores.

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