Density,would help you to determine what layer will be the organic layer. It is important which you recognise whether or not the aqueous layer is above or under the natural layer with inside the separatory funnel, because it dictates which layer is saved and that is ultimately discarded.
Two solvents will stack atop each other primarily based totally on variations in density.In the left isolating funnel, the aqueous layer is at the bottom, which means the natural layer should be much less dense than water.
In the proper isolating funnel, the aqueous layer is at the top, which means the natural layer should be greater dense than water.
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