The SDS-PAGE technique is not usually employed in analyzing oligosaccharides and polysaccharides. While polysaccharides often contain several monosaccharides linked together by the glycosidic bonds, oligosaccharides normally contain only a few sugars, usually 3 to 15, bound together by glycosidic linkages. The Water is a byproduct of the dehydration event that creates glycosidic linkages.
The Monosaccharides, which are little sugar molecules, are the building blocks for both oligosaccharides and polysaccharides. The fundamental distinction between oligosaccharides and polysaccharides is the number of monosaccharides that go into making an oligosaccharide, as opposed to the number of monosaccharides that go into making a polysaccharide.
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