In order to generate several copies of the desired DNA sequence, the PCR procedure includes repeatedly heating and cooling the reaction through a series of temperature variations.
- A partial match between the primer and the template will be stable enough at lower temperatures, allowing for more widespread amplification.
- Your specificity will increase as the temperature rises because longer suitable sequences are needed for the primer to bind to.
- In order to generate several copies of the desired DNA sequence, the PCR procedure includes repeatedly heating and cooling the reaction through a series of temperature variations.
- One significant benefit of PCR is the ability to amplify target sequences from a single copy of the starting material, even when the template is tainted with inhibitors and degraded.
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