Which statement describes how basic DNA technology is used to construct recombinant DNA molecules? (10 points)

A. Scientists use a gel-filled electrical chamber during gel electrophoresis to isolate DNA fragments.

B. Scientists use restriction enzymes to cut DNA molecules and ligation to paste DNA fragments together.

C. Scientists use heat and bacterial
transformation to add novel DNA into bacterial cells.

D. Scientists use high temperatures to separate strands of DNA molecules during polymerase chain reaction.

Question

hellokittyjenni hellokittyjenni

29-11-2021
Biology

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Which statement describes how basic DNA technology is used to construct recombinant DNA molecules? (10 points)

A. Scientists use a gel-filled electrical chamber during gel electrophoresis to isolate DNA fragments.

B. Scientists use restriction enzymes to cut DNA molecules and ligation to paste DNA fragments together.

C. Scientists use heat and bacterial
transformation to add novel DNA into bacterial cells.

D. Scientists use high temperatures to separate strands of DNA molecules during polymerase chain reaction.

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luisvaschetto luisvaschetto · Answer 1 · 2021-12-02T10:36:38+01:00

The statement 'Scientists use restriction enzymes to cut DNA molecules and ligation to paste DNA fragments together' describes the construction of recombinant DNA molecules. This technology is called recombinant DNA.

Recombinant DNA is a genetic engineering approach by which scientists uses specific enzymes to cut and paste nucleotide (DNA) target sequences of interest.

The enzymes that recognize and cut DNA are called restriction enzymes, while enzymes that ligate DNA fragments are called ligase enzymes.

Recombinant nucleotide sequences are placed into specific vectors (e.g., a plasmid) that transport the DNA in a host cell in where this sequence is expressed as protein.

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