Answer: See explanation
Explanation:
The calibration curve is the method used for the determination of the concentration of a substance such that the unknown sample will be compared to some standard samples of the known concentration.
To prepare a standard (calibration) curve for a spectroscopy experiment, start by preparing (multiple solutions) with (different known concentrations). Then, measure the (absorbance) of each solution at (thesame wavelength) and create a plot of (absorbance vs. concentration) for the measured values. Finally, find the best-fit line of the data set.