Respuesta :
B) option is correct
Explanation:
- Hydrogen bonds between base pairs of antiparallel strands are broken: DNA helicase catalyse ATP dependent separation of DNA strand and moves along with template which have 5'-3' orientation
- Single-stranded binding proteins attach to DNA strands: Single strand binding proteins binds with single strands and prevent reannealing of both strands, this binding is sequence independent (cooperatively) and stabilized by ionic interactions
- Primase binds to the site of origin: Primase synthesizes primer during DNA replication which provides 3'-OH group required for phosphodiester bond formation
- An RNA primer is created: DNA bound Dna B protein recruit primase enzyme called Dna G protein which catalyzes synthesis of primer
- DNA polymerase binds to the template strand: DNA dependent synthesis of new DNA is catalysed by a metalloenzyme called DNA polymerase and there are two basic requirements of DNA polymerase: template, which provides information of nucleotide selection and primer which provides 3'-OH group required for phosphodiester bond
The following steps of DNA replication in chronological order.
B. 2, 1, 3, 5, 4
2. Hydrogen bonds between base pairs of antiparallel strands are broken.
1. Single-stranded binding proteins attach to DNA strands.
3. Primase binds to the site of origin.
5. An RNA primer is created.
4. DNA polymerase binds to the template strand.
DNA replication
The DNA is unwound by the help of helicase enzyme. Following this, the bonds between base pairs are broken down to seperate the two strands. Now, to prevent rebinding, Single stranded binding proteins come and stabilize the DNA.
This is followed by activity of primase which syntheisize the RNA primer. Finally, DNA polymerase catalyzes the reaction by adding more nucleotides. And then, ligase joins the strands and completes the process.
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