If you take into account the amount of ATP generated by ATP synthase per molecule of NADH produced in aerobic respiration, the net number of ATP molecules produced by substrate-level phosphorylation, and the fact that NADH molecules produced in the cytoplasm have to be transported into the mitochondria, what is the predicted energy yield of glycolysis in eukaryotic cells?

During glycolysis NAD+ is reduced to NADH by the conversion of glyceraldehyde 3 phosphate to 1,3 bisphosphoglycerate by the catalytic activity of glyceraldehyde 3 phosphate dehydrogenase.

In this reaction 2 molecules of NAD+ is reduced into 2 molecules of NADH. The 2 molecules of NADH enter into electron transport chain to generate 6 molecules of ATP.

when 1,3 bisphosphoglycerate is converted 3 phosphoglycerate by Phosphoglycerate kinase resulting in the formation of 2 molecules of ATP.

From all the above aspects it can be stated that according to the given question the predicted energy yield of Glycolysis in eukaryotic cell is 8 molecules of ATP.