Evaluate Candidate Target Sequences Because the sgRNA sequence determines where Cas9 cuts DNA, it may cut DNA anywhere it encounters a complementary sequence, even in undesired locations. Such unintended cuts are called off-targets. the sgRNA and DNA shown that Cas9 sometimes cuts DNA even when there are mismatches between the potential to be off-target cut sites, means DNA sequences that only partially match the sgRNA have. the potential to be off-target cut sites, although the PAM sequence is still required. In general, the more mismatches there are between a DNA sequence and the sgRNA, the less likely Cas is to cut. 1. Develop three criteria that could be used to rank the candidate target sites according to their as well as the information you considered fresults you have collected in your BLAST search select criteria. For each criterion, explain how the readings and focus questions as you By the cvaluation of the con it will help you evaluate a potential target site. these criterias, researchers are atidate target sites basedion effective sites for theraputir able to select the Safest and risies of unintended mutations use. This can help minimice the benefits of genome editing and other effects. While marimizi and other diseases. 2. Use your criteria to evaluate and rank the candidate target sites you selected in Part 1, step 3. Write a claim about which candidate target site is the best option for your therapeutic application, based on your evaluation, and provide reasoning.