Which one of the following is an example of epigenetic inheritance: A) the inheritance of nuclear UNA from one cell generation to the next B) the inheritance of extra-chromosomal DNA from one cell generation to the next C) the inheritance of mRNA from one cell generation to the next D) the inheritance of chromatin states from one cell generation to the next E) the inheritance of acetylated DNA 6. Which of the following tools of recombinant DNA technology is incorrectly paired with its use? There may be more than one correct answer. (A) restriction enzyme: sequence-specific cutting of DNA (B) DNA ligase: joining pieces of DNA (C) Taq DNA polymerase: amplification of a specific sequence of DNA (D) DNA gel electrophoresis: Northern blot (F) western blot: detection of proteins (G) reverse transcriptase: production of genomic libraries 7. Suppose you have identified a mutant strain of yeast that cannot grow without leucine added to the growth medium. You know that the leu" phenotype results from a single mutation, but you do not know where in the yeast genome the mutation occurred. Which of the following strategies would you use to clone the wild-type gene corresponding to the mutant allele? Only one answer correct will be considered correct. (A) PCR amplification of the gene using flanking primers (B) screening of a yeast-cDNA expression library using an antibody probe (C) complementation of the leu" phenotype with a yeast genomic plasmid library (D) hybridization of a DNA probe to a southern blot (E) hybridization of a DNA probe to a northern blot (F) screening of a yeast-genomic lambda phage library using a DNA probe 8. At the end of the paper they published in 1953, Watson and Crick said that an aspect of their proposed DNA structure "immediately suggests a possible copying mechanism for the genetic material." It was a vague statement, not supported by evidence or experiment at they time, but nonetheless, it conveyed confidence that the structure makes the method of replication obvious. What is the primary structural feature of DNA that lends itself to replication?