A 2.00-mL solution of apotransferrin was titrated as illustrated in Figure 18-11. It required 163 mL of 1.43 mM ferric nitrilotriacetate to reach the end point.
(a) Why does the slope of the absorbance versus volume graph
change abruptly at the equivalence point?
(b) How many moles of Fe(III) (5 ferric nitrilotriacetate) were
required to reach the end point?
(c) Each apotransferrin molecule binds two ferric ions. Find the
molar concentration of apotransferrin in the 2.00-mL solution.